Journal: Open Forum Infectious Diseases
Article Title: A Rapid Host Response Blood Test for Bacterial/Viral Infection Discrimination Using a Portable Molecular Diagnostic Platform
doi: 10.1093/ofid/ofae729
Figure Lengend Snippet: Biomeme M1/Franklin HR-B/V workflow The system consists of 4 components: an M1 RNA 2.0 sample prep cartridge, 3 Go-Strips panels (A, B, C), the Franklin three9 real-time polymerase chain reaction (PCR) thermocycler, and a smartphone. The M1 sample prep cartridge allows rapid nucleic acid extraction without the need for any equipment. Each Go-Strip consists of 3 connected PCR tubes containing lyophilized master mix, multiplexed primers, and probes for 3 targets. Each handheld unit is equipped with 9 sample wells, enabling simultaneous quantitative detection of up to 27 targets per PCR run across 3 different color channels including green (FAM/SYBR), amber (Jun/TexRedX), and red (ATTO647N/Cy5). The thermocycler is just under 3 lb (1.36 kg); battery-operated for maximum portability, enabling a full day's work on a single charge; and provides results in less than an hour. The smartphone uses an intuitive app, the Biomeme Go, which pairs wirelessly with the Franklin thermocycler to easily run, monitor, analyze, and share the PCR data.
Article Snippet: Sample was pumped through the Biomeme M1 sample prep column, which contains silica membranes, a barbed tip, and Luer lock for attachment to a 1-mL syringe.
Techniques: Sample Prep, Real-time Polymerase Chain Reaction, Extraction, Stripping Membranes, Battery